LIM KIM CHOOI, PATRICIA

Designation/Title: Professor; Deputy Director, Commercialisation & Support, IRDI
Department: Pathology, School of Medicine, International Medical University
Tel: 03-27317586
Fax: 03-86567229
E-mail : This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 

Education and Training

PhD University of Malaya,
1992
MSc
University of Malaya,
1984
Dip. Ed. University of Malaya,
1977
BSc (Hons)
University of Malaya,
1976
BSc
University of Malaya,
1975

 

Research interests:

  • Tropical Medicine
  • Parasitology
  • Molecular Biology
  • Infectious Diseases

 

Research projects/grants:

National Research & Development Grants (as Principal Investigator):

  • Production of monoclonal anti-bodies against merozoite antigens of Plasmodium falciparum and P.cynomologi, MPKSN (R&D), - RM 19,820 (1984-1985).
  • Identification of disease causing vectors by bloodmeal test, MPKSN (R&D)- RM3,000 (1988).
  • Studies on monoclonal antibodies produced against P. falciparum and P. cynomologi. MPKSN (R&D) – RM 6,130 (1988).
  • Development of ELISA for bloodmeal identification of mosquito vectors, MPKSN (R&D) - RM 50,510 (1989-1990).
  • Isolation of clones from local isolates of P.falciparum for biochemical, immunological and drug resistance studies. MPKSN (R&D) – RM107,795 (1989-1991).
  • Production and use of DNA probes against P. falciparum and P. cynomologi, IRPA (R&D) – RM 81,000 (1991-1993).
  • IMR-JICA Collaborative Project on Tropical Diseases (DNA Sequencing studies on P. vivax mitochondrial genes), JICA Grant- RM 200,000 (1993-1995).
  • Studies on protective epitopes and mitochondrial DNA of Plasmodium parasites, IRPA (R&D) – RM 151,750 (1994-1995).
  • Rapid detection of mutations in chloroquine and pyrimethamine-resistant Plasmodium falciparum by polymerase chain reaction, IRPA (R&D) – RM 33,500 (1995).
  • Molecular approaches to the study of drug-resistant malaria parasites, IRPA (R&D)- RM 90,000 (1996- 1997).
  • Regional Collaboration for rapid malaria diagnosis in Southeast Asia and China. (as collaborator), WHO Grant - RM 100,000 (1995-1996).
  • Topdown National Biotechnology project “Molecular approaches to the development of biomarkers for disease and disease susceptibility and development of appropriate techniques for diagnosis, Ministry of Science Malaysia (NBD) / IRPA – RM 1,600,000 (1996-2000).
  • Development of in vitro molecular assays for screening of antimalarial compounds. IRPA (R&D) – RM 90,000 (1999-2000).
  • Topdown National Biotechnology Project ” Molecular Studies on disease biomarkers and pathogenesis of cancer: Colorectal carcinoma as a model”, Ministry of Science Malaysia (NBD) / IRPA, - RM 2,527,575 (2002-2005).
  • Prioritised Research project: Mechanism of action of antivirals against nasopharyngeal carcinoma at the molecular level. IRPA (Prioritised Research) – RM 1,000,000 (2003-2007).
  • Technofund Project: A multiprong approach for the detection of Salmonella based on novel DNA Sequences. (UM-IMU-MBDR collaboration), (Principle investigator: MBDr), TECHNOFUND Grant , MOSTI – RM 1,600,000 (2007-2010).
  • Characterisation of cyst and trophozoite proteins of Acanthamoeba castellani and A. polyphaga isolates by SDS-PAGE and two-dimensional (2-D) gel electrophoresis, IMU Internal grant – RM 20,000 (July 2011-June 2012).
  • Determination of heavy metals and fungi associated with raw and commercial edicle bird nests (EBN), IMU internal grant – RM 20,000 (February 2012 – January 2013).
  • Molecular detection of dengue virus and Wolbachia in Aedes larvae collected from ovitraps in urban areas in Kuala Lumpur. IMU Internal Grant – RM 20,000 ( August 2013-July 2014). .
  • Expression and roles of Interferon Regulatory Factor-5 (IRF5) in dust mite induced asthmatic and non- asthmatic patients – RM 40,000 (August 2013 – September 2015).
  • Identification of parasites in soil samples collec ted from Signy Island, Antarctica (polar) and Lang Valley, Malaysia (tropics), IMU Internal grant, December 2015- March 2016; RM 7,500.
  • Epidemiology of dengue in Malaysia and he dynamics of dengue-virus-vector interactions, IMU Internal grant, December 2015- May 2018, RM 60,000.
  • Influence of dengue awareness on Aedes larvae incidence in childrens’ homes in Kuala Lumpur. IMU Cares Internal Grant, Mar 2016 – Feb 2017. (RM 2143)

 

International and National Research and Development Grants (As Collaborator)

  • WHO/TDR Grant for 'In vitro cultivation of Brugia malayi and Brugia pahangi' (ID 790015), US$5,000 (1981). PI: Prof Mak Joon Wah
  • WHO/VBC Grant for 'Provision of blood meal identification of vectors' - US$5,000 (1987). PI: Prof Mak Joon Wah
  • WHO/TDR Grant for 'Testing filaricides against Brugia malayi in leaf-monkeys' - I.D. 87013, US$49,700 (1987-1989). PI: Prof Mak Joon Wah
  • WHO/TDR Grant for 'Antifilarial drug testing in vitro and in vivo - I.D.910334, US$20,000 (1992/93). PI: Prof Mak Joon Wah
  • Epidemiology of malaria among Orang Asli and rural communities' - RM37,721 (1988-90). PI: Prof Mak Joon Wah
  • Development and use of immunodiagnostic tests for parasitic diseases' - RM20,500 (1988). PI: Prof Mak Joon Wah
  • Production, characterization and use of Toxocara monoclonal antibodies (R&D IMR 91-23)' - RM 20,500 (1991); RM21,500 (1992); RM21,500 (1993). PI: Prof Mak Joon Wah
  • Seroepidemiology and drug sensitivity studies in malaria (R&D IMR 91-26)' - RM20,000 (1991); RM20,00 (1992). PI: Prof Mak Joon Wah
  • Production of fusion proteins of Toxocara canis infective larvae and adult worm, primarily as antigen source in the immunodiagnosis of toxocariasis (R&D IMR 91-27) - RM37,150 (1991); RM13,500 (1992). PI: Prof Mak Joon Wah
  • Development and use of monoclonal antibodies for parasitic diseases with special emphasis on malaria, filariasis, toxocariasis and toxoplasmosis (National Biotechnology Programme - Sub-programme Medical Biotechnology, B.U.2) - RM58,000 (1991); RM34,568 (1992); RM40,000 (1993); RM60,000 (1994). PI: Prof Mak Joon Wah
  • Occurrence of Acanthamoeba spp. and bacterial endosymbionts in dust samples collected from IMU dental clinic, Bukit Jalil cmpus and two other dental clinics in Kuala Lumpur, IMU Internal grant RM20,000, July 2011-June 2012. PI: Assoc Prof Dr. Muneer G. Babar.
  • Isolation and identification of mites associated with raw and commercial edible bird nests (EBN), IMU internal grant RM 20,000, February 2012 – January 2013. PI: Dr. Wong Shew Fung (IMU).
  • Systemic delivery of siRNA-based therapeutics using functional single wall nanotubes (SWNTs), 2011-2014, MOSTI (NND), RM 329,800. PI: Assoc Prof Leong Chee Onn.
  • Characterizing Host Parasite Metabolic Interaction of Toxoplasma gondii infection in murine model: A systems biology approach, September 2012-August 2014. E-Science grant No. 02-02-09-SF0033, RM 234,000. PI: Dr Ivan Yap Kok Seng.
  • Production of monoclonal and polyclonal antibodies against surface protein of Salmonella typhi strain Ty2. IMU Internal grant, Nov 2011-Nov 2013, RM 40,000, . PI: Prof Ong Kok Hai.
  • Role of biomarker Cytokeratin-18 (CK-18) in severity assessment and stratification of patients with non-alcoholic fatty liver disease IMU Internal Grant, Feb 2013-Jan 2014, RM 20,000, PI: Prof Dato’ Kew Siang Tong
  • Rapid identification of new molecular targets for treatment of endometrial cancer. ERGS Grant, May 2013- April 2015, RM 227,000.00, PI: Dr Chan Kok Keong.
  • Flagship FP1213E036-Sub-Project 4: Biomarkers of polar and lower latitudes microbes in relation to heavy metal stress, August 2014-July 2016, RM 819,902, PI: Prof Chu Wan Loy,
  • System biology comparison between mice with single and co-infection from Toxoplasma gondii and/or Trypanosoma evansi, FRGS Grant, Dec 2015-May 2018, RM 162,000, PI: Dr Ivan Yap Kok Seng.

 

Supervision of Postgraduates (MSc/PhD)
(Last 5 years – 2012-2016)

  • Expression and roles of Interferon Regulatory Factor-5 (IRF5) in dust mite induced asthmatic and non-asthmatic patients  - Voon Yit Nee, MSC Project, 2013-2015.

  • Tan Yuen Fen. Systemic delivery of siRNA-based therapeutics using functionalized single-walled carbon nanotubes (SWNTs).  Doctor of Philosophy Science in Medical and Health Science (by research), IMU, from February 2014 – Jan 2020.

  • Nur Alia Binti Johari. Epidemiology of dengue in Malaysia and the dynamics of dengue virus-vector – host interactions. Doctor of Philosophy Science in Medical and Health Science (by research), IMU, from December 2015- May 2018.

 

Professional affiliations:

  • Malaysian Society of Parasitology & Tropical Medicine

 

Academic Awards:

  • ...

Recent publications
(last 5 years – 2011 -2015)

2011

  • Kok YY, Chu WL, Phang SM, Mohamed SM, Naidu R, Lai PJ, Ling SN, Mak JW, Lim PKC, Balraj P, Khoo ASB (2011). Inhibitory activities of microalgae extracts against Epstein-Barr virus DNA release from lymphoblastoid cell. Journal of Zheijang University -Science B (Biomed & Biotechnol) 12(5): 335-345. (NA).
  • Lim PKC (2011). Editorial. The role of academic publications in community service. IeJSME 5(1): 1. (NA).
  • Ong RCB, Lim PKC, JW Mak (2011). Detection of glycoproteins from human erythrocytes of different ABO blood groups infected with Plasmodium falciparum. IeJSME 5(2): 18-28. (NA).

 

2014

  • Yap RMD, Lim PKC, Chan LL, Wong SF, Mak JW. Characterization of cyst and trophozoite proteins of environmental isolates of Acanthamoeba castellani by two-dimensional gel electrophoresis. Southeast Asian Journal of Tropical Medicine and Public Health 2014; 45: 249-258. (Q3, IF 0.66, HI 33).
  • Kew PE, Wong SF, Lim PKC, Mak JW. Structural analysis of raw and commercial farm edible bird nests. Tropical Biomedicine 2014; 3(1): 63-76. (Q3, IF 1.06, HI 16).
  • Kok YY, Chu WL, Phang SM, Shar Mariam M, Naidu R, Lai PJ, Ling SN, Mak JW, Lim PKC, Balraj P and Khoo ASB. Inhibitory activities of microalgal extracts against Epstein-Barr Virus (EBV) antigen expression in lymphoblastoid cells. Arch. Biol. Sci., Belgrade, 2014; 66 (3), 1009-1024. DOI:10.2298/ABS1403009K. (Q3, IF 0.65, HI 12).
  • Naidu R, Lim VN, Mak JW, Moissinac K, Jamal ARA, Rose IM, Gul YGA, Lim PKC, Tan GJS. Protein Expression of STAT3, pSTAT3, MMP-7 and VEGF in colorectal adenocarcinoma: An immunohistochemical study. Journal of Cancer Therapy, 2014; 5: 1175-1185. (NA).
  • Chen JXJ, Lim PKC, Wong SF, Mak JW. Determination of the presence and level of heavy metals and other elements in raw and commercial edible bird nests. Malaysian Journal of Nutrition 2014; 20(3): 377-392. (NA).

 

2015

  • Chu WL, Tan EL, Ambu S, Leong CO, Nadarajah VD, Lim PKC, Wong SF, Khor GL, Menke JM, Mak JW (2015). Institute for Research, Development and Innovation (IRDI) in the International Medical University (IMU). The Malaysia-Japan Model of Technology Partnership, pp. 387-395, Springer Japan. (NA).
  • Lim PKC, Yamasaki, H, Mak JW, Wong SF, Chong CW, Yap IKS, Ambu S, Kumarasamy V. Field evaluation of a rapid diagnostic test to detect antibodies in human toxocaraisis. Acta Tropica 2015, 148: 32-37. http://dx.doi.org/10.1016/j.actatropica.2015.04.011. (Q1, IF 2.56, HI 69).
  • Chen JXJ, Wong SF, Lim PKC, Mak JW. Culture and molecular identification of fungal contaminants in edible bird nests. Food Additives & Contaminants: Part A · Online 4 November 2015. DOI: 10.1080/19440049.2015.1101494 (SCI IF: 1.08, H-index 33; Tier Q1).
  • Pei Ern Kew, Suk Jiun Ling, Shew Fung Wong, Patricia Kim Chooi Lim, Joon Wah Mak. Isolation and identification of mites associated with raw and commercial farm edible bird nests. Tropical Biomedicine (accepted for publication) [BMedSc BMS I-01/2012(03)]
  • Learning for the Future – The IMU Experiment (2015). 261 pp. ISBN: 978-967-1370407; Editors: PKC Lim and JW Mak.


2016

  • Kho MT, Chong CW, Lim ESH, Ismail NH, Draper LO, Yam WK, Lim PKC, Mak JW, Yap IKS. Systems biology analyses of the dynamic host response to Toxoplasma gondii infection in a murine model. Parasitology Open 2016, 2 (e9): 1-13
  • Lim PKC, Babar MG. Service Learning in Health Professions Education for the 21st Century. IeJSME 2016, 10(2): 1-2.
 

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